Table 2. Oligonucleotide primers and probes.

Oligonucleotide	Sequence (5′ → 3′)
IS6110c_Forward	GATCCTGCGAGCGTAGGCGTCGGTGACAAAGGCCACGTAG
IS6110c_Reverse	CTGATCCGGCCACAGCCCGTCCCGCCGATCTCGTCCAGC
IS6110c_Probe	CGAACCCTGCCCAGGTCGACACATAGGTGAGGTC(F)(H)C(Q)ACCCACAGCCGGTTA-Spacer C3
IS1081a_Forward	CAGTAGTGGGCGGTCATCGCGTGATCCTTCGAAACGACC
IS1081a_Reverse	CTCGCCTGTGCGAGTTGGTCAGCCAGAAGCTG
IS1081a_Probe	CGATAAGATGAGAAGAGGTCATTGCGTCATT(F)(H)C(Q)TCGATTGACTTTTGCT-Spacer C3

The oligonucleotides chosen for amplification and detection of IS6110 and IS1081 are shown in Table 2. F = dT-FAM, H = tetra hydrofuran and Q = dT-Black Hole Quencher 1.