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. Author manuscript; available in PMC: 2014 Aug 19.
Published in final edited form as: Mass Spectrom Rev. 2014 May-Jun;33(3):157–182. doi: 10.1002/mas.21389

Figure 8.

Figure 8

MALDI-MS/MS analysis of the mitochondrial protein acyl-CoA dehydrogenase (long-chain specific; ACADL_RAT) modified on Cys-166 with different endogenous electrophilic lipoxydation products . ARP was used for the chemoselective tagging of proteins with reactive carbonyl/keto groups. After trypsin digestion, ARP-tagged peptides were enriched using immobilized monomeric avidin beads and submitted to MALDI-CID (1keV) MS/MS analysis using an Applied Biosystem 4700 ToF-ToF instrument. “Peptide” ions as well as the ARP-derived “non-sequence” ion are annotated. The asterisks denote fragment ions that retained the lipoxidation-derived 2-alkenal modification during CID-MS/MS. Reproduced from Chavez et al (2011) with permission from Elsevier, copyright 2011.