Fig. 1.

Human BMSCs express histamine receptors

Immunostaining of cultured human BMSCs using specific antibodies to recognize the H1(A), H2 (B), H3 (C) and H4 (D) receptors. The green fluorescence is due to the Alexafluor-488 secondary antibody. The bar represents 25 µm. The upper right insets demonstrate RT-PCR results using receptor specific primers. The arrows point at the expected size of the receptor proteins (all around 500 bases).

The H1, H2 and H4 receptors are present with both techniques, while the H3 does not seem to be expressed.