Fig. 2.

Reduction of Mb by Asc. (A) An illustration of reduction of 10 μM Mb(Fe³⁺) to Mb(Fe²⁺) by 10 mM ascorbate at 37 °C under anaerobic conditions, which was indicated by a shift of the heme Soret band from 409 nm to 434 nm. (B) The second order rate constant k_Mb at 37 °C was determined as (8.7 ± 0.3) × 10⁻² M⁻¹s⁻¹ (n=5) by the plot of the pseudo first order rate constant k’_Mb versus Asc concentration (10-100 mM).