Table 5. Comparisons of relative gene expression fold between gene expression data and qPCR results.
Name | gene ID | Fold change at 6/24/48 h (gene expression data) | qPCR fold change at 6/24/48 h | ||||
attacin | Unigene12874 | 15.2 | 4.7 | 3.9 | 210.8±59.0** | 52.6±15.8** | 25.1±4.2** |
defensin | CL7841.Contig1 | 15.9 | 3.4 | 6.0 | 220.3±60.5** | 45.1±12.9** | 63.2±16.1** |
diptericin | Unigene13439 | 12.8 | 9.8 | 5.7 | 180.5±51.4** | 112.3±30.8** | 62.9±18.5** |
muscin | Unigene29893 | 8.3 | - | 1.9 | 55.0±16.2** | 16.0±4.3** | 26.0±7.6** |
prophenoloxidase | Unigene28739 | - | - | 10.9 | 42.3±12.1** | 51.6±10.9** | 116.7±32.2** |
PGRP-SD | CL486.Contig1 | 4.2 | 1.4 | 1.6 | 7.8±2.4** | 1.5±0.3** | 1.1±0.3* |
HSP67B2 | CL8219.Contig1 | - | 1.1 | 1.2 | 1.0±0.3 | 0.9±0.2 | 1.0±0.2 |
eiger | CL358.Contig2 | - | 1.5 | 3.5 | 2.5±0.7** | 3.9±1.1** | 4.8±1.1** |
metallothionein | Unigene13124 | - | 1.2 | - | 1.0±0.3 | 1.0±0.2 | 3.1±0.9** |
SOD | CL6208.Contig1 | - | - | 1.9 | 1.9±0.5 | 1.1±0.3 | 2.1±0.5* |
Significant differences between the challenged and control group are indicated with * at P<0.05 or ** at P<0.01.