Table 2. Cross reactivity pattern of KRAS mutation detection assay.
KRAS mutation detection assay | ||||||||
G12D | G13D | G12R | G12C | G12S | G12V | G12A | ||
20 ng DNA input | LS174T (c.35G>A; p.G12D) | −1.6 | — | — | — | — | — | — |
HCT116 (c.38G>A; p.G13D) | — | -1.7 | — | — | — | — | — | |
HUPT3 (c.34G>C; p.G12R) | — | — | -0.5 | 14 | — | — | — | |
KYSE410 (c.34G>T; p.G12C) | — | — | 5.8 | 0.9 | — | — | — | |
A549 (c.34G>A; p.G12S) | — | 6.4 | 9.3 | 5.5 | −2.9 | — | — | |
SW403 (c.35G>T; p.G12V) | 4.5 | — | — | — | — | 0.6 | — | |
RPMI8226 (c.35G>C; p.G12A) | 0.5 | — | — | — | — | — | −18.1 |