Figure 1.

A. A hydropathical profile predicted using the Kyte-Doolittle method with a window size of 9 amino acid residues; B. Computer-assisted prediction of B cell epitopes from human SOX2; C. Peptides synthesized for screening B cell epitopes from SOX2; D. Human SOX family members that share homology with the identified B cell epitopes are highlighted. Initial alignment of SOX proteins and B-cell epitopes was performed using ClustalW2 alignment tool from EMBL-EBI website (www.ebi.ac.uk/tools/msa/clustalw2). Accession numbers for SOX proteins are provided in parentheses.