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Validation of autoAb responses against B cell epitopes derived from SOX2 using a seropositive sample (#KHA-71) known to contain high titer autoAb against SOX2 as control, by ELISA (A) and by Western blot on the full-length SOX2 protein (B). In the Western blot, about 6 μg lysates from HEK293T cells, labeled as H, were used as negative controls side by side with 200 ng purified recombinant SOX2 protein, labeled as S.
