Table 1. List of potential tyrosine-sulfated proteins in cow RPE.
Protein name | Mascot Score | RefSeq# | Coverage % | |
* | Opticin | 605 | 45429965 | 26 |
* | Lumican | 630 | 27806853 | 31 |
* | Vitronectin | 313 | 78045497 | 15 |
* | Heparin cofactor II | 722 | 76639676 | 26 |
* | Similar to Amyloid beta A4 protein precursor | 851 | 76607645 | 22 |
1. | Fibrinogen, gamma | 1093 | 27806893 | 43 |
2. | Complement C4 | 978 | 76650940 | 10 |
3. | Dickkopf related protein 3 | 863 | 76635678 | 28 |
4. | Fibulin 2 | 774 | 76649536 | 13 |
5. | Fibrinogen beta | 657 | 76638241 | 49 |
6. | Secreted frizzled related protein | 662 | 27806625 | 39 |
7. | Tubby like protein 1 | 557 | 76672237 | 54 |
8. | Spondin 1 | 480 | 27807443 | 14 |
9. | Retinol Binding protein 3 (IRBP) | 467 | 27806445 | 9 |
10. | Fibroblast growth factor | 381 | 27806627 | 31 |
11. | Fibrinogen alpha | 319 | 75812954 | 10 |
Sixteen proteins were identified in cow RPE by MALDI-MS analysis of SDS-PAGE gel slices of PSG2 immunoaffinity column eluent. The asterisks mark those proteins at 75 and 50 kD, shown in Figure 2C. NCBI reference sequence database ID numbers (RefSeq#) and percent sequence coverage (coverage %) are also indicated for each protein. Inclusion in this table does not confirm that the protein is tyrosine-sulfated. Some of the proteins may be isolated by the affinity column as a result of their interaction with sulfated proteins.