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. 2014 Aug 19;9(8):e105013. doi: 10.1371/journal.pone.0105013

Figure 2. Immunoblotting of F4ab/acR+ BBMVs with F4ac fimbriae (lane 2 and 4) or anti-MUC13 antibodies (lane 3 and 5).

Figure 2

Proteins were separated under reducing (A) and non-reducing (B) conditions. F4ac fimbriae bound to the F4-specific high molecular weight glycoproteins (only present in F4R+ pigs) and several non-specific F4-binding bands <50 kDa (present in F4R+ and F4R− pigs) [20]. Anti-MUC13 antibodies recognized BBMV protein bands of 55, 47, 34 and <25 kDa under reducing and non-reducing conditions, but bands of 200, 110 kDa only under reducing conditions. Lane 1  =  protein standards.