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. 2014 Aug 19;9(8):e105013. doi: 10.1371/journal.pone.0105013

Figure 4. Absence of F4ac fimbriae binding to immunoprecipitated intestinal MUC13.

Figure 4

Intestinal MUC13 was purified from 1 mg F4ab/acR+ brush border membrane vesicles (BBMVs) by immunoprecipitation with anti-MUC13 antibodies and protein A sepharose. The eluate (lane 2) and the non-precipitated fraction (lane 3) were immunoblotted with anti-MUC13 antibodies (A) or with F4ac fimbriae (B). Immunoprecipitation enriched a protein with a band of 110 kDa but this was not recognized by F4ac fimbriae. The F4-specific high MW glycoproteins were found in the non-precipitated fraction. Lane 1  =  protein standards. Arrows: position of the high MW glycoproteins.