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. 2014 Aug 19;9(8):e105231. doi: 10.1371/journal.pone.0105231

Figure 9. Protease activity.

Figure 9

Conditioned media of AP-1 cells in the 7th and 13th passages were analyzed by zymography. Gelatinolytic bands corresponding to MMP2 and MMP9 are observed in both groups. Both active and latent forms of MMP2 are observed. MMP9 latent form seems to be secreted by cells in the 13th passage. MMP2 and MMP9 positive controls (Std MMP) are included. Zymographic experiments were carried out at least three times with consistent results.