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. 2014 Aug 19;9(8):e105168. doi: 10.1371/journal.pone.0105168

Figure 4. The effect of kinase inhibitors on ET-1 and mechanical stretch-induced BNP gene expression.

Figure 4

ERK inhibitor PD98059 (10 µM) (A, D), p38 inhibitor SB203580 (10 µM) (B, E) or PKA inhibitor H89 (10 µM) (C, F) were added to culture medium of neonatal rat cardiomyocyte cell cultures 2 hours prior to 1 h ET-1 stimuli (100 nM; A–C) or cyclic mechanical stretching (D–F). DMSO was used as a control. The mRNA levels were normalized to 18S quantified from the same samples and the mRNA levels are presented relative to control cells. The results represent mean ± SEM (n =  8–18) from at least 3 independent experiments. *P<0.05; **P<0.01; ***P<0.001 compared to control.