Figure 2. Conservation of HR2 and C-terminal region of Xbp1^unpsliced.

(A) Kyte and Doolittle hydrophobicity plot of Drosophila Xbp1^unspliced, indicating the existence of two hydrophobic regions, HR1 and HR2. The horizontal red line indicates a score of 1.8. (B) Local amino acid sequence alignment of HR2 using several species. Amino acids that are fully, strongly or weakly conserved are indicated. The hydrophobicity of each amino acid is indicated by a color code. (C) Local amino acid sequence alignment of the C-terminal translational pausing region. Amino acids that are fully, strongly or weakly conserved are indicated.