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. 2014 Aug 19;9(8):e105588. doi: 10.1371/journal.pone.0105588

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© 2014 Coelho et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Sequencing of single colonies obtained after cloning of Excision101 specific cDNA. The absence of the intron in colonies 1–4 demonstrates that Excision101 mRNA is spliced by Ire1. Percentage of colonies containing Xbp1^spliced form in Excision101 or CyO control chromosome. The total number of colonies sequenced was 38 for Excision101 and 37 for the CyO control.