Figure 3. Comparable systemic immune response between C57BL/6 and IL-17RA^−/− mice.

(A) Flow cytometric analysis of splenic cells from C57BL/6 (n=9) and IL-17RA^−/− (n=10) was performed at Day 14 to determine the frequency of GC B cells (B220⁺GL-7⁺CD95⁺) and plasma cells (B220^loCD138^hi). Numbers in the dot plot represent percentage of cells. (B) At Day 14, serum titers of mouse anti-rabbit total IgG, IgG1 isotype, and IgG2a isotype were measured by ELISA in C57BL/6 (n=9) and IL-17RA^−/− (n=10) mice. Flow cytometric analysis of splenic cells from C57BL/6 (n=9) and IL-17RA^−/− (n=10) was performed at Day 14 to determine the frequency of (C) effector CD4⁺ T cells (CD4⁺CD62L^loCD44^hi) and (D) natural T_regs (CD4⁺CD25⁺Foxp3⁺). Each dot represents individual mice, and the horizontal bars indicate mean for each group. Data are pooled from two independent experiments. (E) At Day 14, splenocytes were stimulated with PMA/ionomycin for 4 h and intracellularly stained for IL-17A and IFN-γ, followed by FACS analyses (gated on CD4⁺ cells). Numbers in the dot plot represent percentage of cells.