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. 2014 Sep;96(3):419–426. doi: 10.1189/jlb.2AB0314-145R

Figure 6. Evaluation of Cebpa-hCD4 transgene RNA expression in nonhematopoietic tissues and in marrow subsets.

Figure 6.

(A) Total RNA from bone marrow or from indicated tissues was assessed for hCD4 RNA expression by qRT-PCR, normalized to ribosomal protein S16 RNA expression. Relative hCD4 expression between all samples is shown (n=4 mice/line). (B) Total RNAs from indicated marrow hematopoietic subsets were analyzed similarly. Although plotted separately, relative hCD4 expression for Lines 1 and 2 samples were all compared in simultaneous analyses—note differences on ordinate scale (n=4). (C) These hematopoietic RNA samples were also analyzed for relative endogenous Cebpa RNA expression (n=4).