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. 2014 Sep;96(3):397–410. doi: 10.1189/jlb.1A0913-500RR

Figure 1. CD8+ T cells proliferate but fail to express T-bet or Eomes upon self-antigen encounter.

Figure 1.

CD8+ CD90.1+ Gag-specific T cells were adoptively transferred into naive (B6), immunizing (B6+FBL), or tolerizing (Alb:Gag) environments. (A) CFSE dilution by transferred T cells was assessed 3 days later and is displayed in overlayed histograms. (B) Real-time PCR analysis of tbx21, eomes, ifng, cxcr3, gzmb, fasl, and pdcd1 mRNA expression in FACS-purified T cells, 3 days after transfer into a naive, immunizing, or tolerizing environment. Data are presented as relative expression versus β-actin control, and error bars are sd. (C) T-bet, Eomes, and CXCR3 protein expression in T cells, 3 days after adoptive transfer, is compared directly by overlaid histograms from naive, immunizing, or tolerizing environments. Data are representative of three experiments, each with three recipients/group.