Figure 4. 1MT treatment enhances AM expression of TNF-α and IL-6.

Mice were pretreated with 1MT or con water for 3 days before intranasal infection with 10³ PFU of X31 or mock-infected. AMs were isolated from BAL collected 24 hpi. Isolated AMs were cultured for an additional 48 h, and levels of (A) IL-6 or (B) TNF-α were evaluated in the supernatant through ELISA. Significance was assigned using a Student's t-test comparing 1MT with con treatment. Graphs show the mean and sem from two combined independent experiments (n=7–8; *P<0.05).