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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Immunol Methods. 2014 Mar 28;0:31–43. doi: 10.1016/j.jim.2014.03.017

Table 3.

Summary of EP4 and EP5 grading.

EQAPOL ELISPOT EP4 & EP5 Site 001 Site 003 Site 005 Site 007 Site 009 Site 010 Site 012 Site 013 Site 014 Site 015 Site 103 EP EPs Average
Criteria Max Points EP# Points Earned Average
Timeliness 10 4 10 10 10 10 10 10 10 10 10 10 10 10 8.64
5 10 10 10 0 0 ^ 0 10 10 10 10 10 7.27
PBMC Handling 9 4 7.5 9 9 6 4.5 9 9 9 4.5 7.5 9 7.64 7.57
5 3 9 9 4.5 7.5 7.5 9 6 9 9 9 7.5
Background 9 4 9 6 9 9 9 9 9 0 9 9 9 7.91 7.64
5 9 3 9 9 9 9 9 0 9 6 9 7.36
Accuracy 54 4 45 36 54 0 45 45 54 9 18 54 54 37.64 40.91
5 45 54 54 54 45 54 54 0 18 54 54 44.18
Precision 18 4 18 15 18 3 18 12 18 9 15 18 18 14.73 14.45
5 18 15 18 18 15 18 18 3 6 12 15 14.18
Total Performance Score 100 4 89.5 76 100 28 86.5 85 100 37 56.5 98.5 100 77.9 79.20
100 5 85 91 100 85.5 76.5 88.5 100 19 52 91 97 80.5
Performance Rating 4 Good Good Excellent Poor Good Good Excellent Poor Poor Excellent Excellent
5 Good Excellent Excellent Good Good Good Excellent Poor Poor Excellent Excellent