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. Author manuscript; available in PMC: 2015 Sep 5.
Published in final edited form as: Neurosci Lett. 2014 Apr 3;579:190–194. doi: 10.1016/j.neulet.2014.03.062

Figure 1.

Figure 1

The schematic experimental schedule for the induction of CHS. The mice were sensitized by the topical application of 25 µL of 1% SADBE in acetone to abdominal skin once daily for three consecutive days. Five days later, the right cheek was challenged either with a topical application of 25 µL of 1% SADBE in acetone (“CHS mice”) or the acetone vehicle alone (“control mice”) once a day for 2 consecutive days. Twenty-four hours later after the second challenge, a pruritic or algesic chemical was intradermally injected into the right cheek.