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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Mech Dev. 2014 Feb 19;0:177–188. doi: 10.1016/j.mod.2014.02.003

Figure 6. Loss of crb2a results in a decrease of 4xGTIIC:d2GFP expression in the neural retina at 48 hpf and at 24, 36, 48 hpf in the RPE.

Figure 6

(A–F′) Neural retina and RPE 4xGTIIC:d2GFP expression in crb2a +/? and crb2a −/− embryos. (G–L‴) Immunofluorescence of Yap (green) and Crb2a (red) in crb2a +/? and crb2a −/− embryos. Arrow indicates Yap positive cells in the crb2a +/? neural retina that do not appear in crb2a −/−. (M,N) Quantification of d2GFP pixel intensity in the (M) neural retina and (N) RPE of crb2a +/?/crb2a −/− embryos at 24 (n=46/n=13), 36 (n=32/n=28), and 48 (n=26/n=23) hpf. Error bars represent S.E.M. p=unpaired t-test with equal S.D. Scale bar=50μm in A,C, and E.