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Knockdown activity of the shRT5 variants. The knockdown activity of the guide strand on Luc-sense (upper panel) and passenger strand on Luc-anti-sense (lower panel) of the shRT5 was determined by cotransfection of a luciferase reporter encoding the sense and anti-sense target sequence, respectively, in HEK 293T cells. We performed three independent transfections, each in duplicate, and standard deviations were calculated. See Figure 3 for details.
