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. 2014 Sep;20(9):1410–1418. doi: 10.1261/rna.043950.113

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© 2014 Herrera-Carrillo et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 8. — Processing of shRNA in DCR-deficient cells. (A) The indicated shRNA constructs (5 µg) were transfected in HCT116 wild-type (WT) or mutant (M) Dicer cells. Total RNA was isolated and analyzed by Northern blot analysis. HCT116 mutant Dicer cells encode an inactive Dicer with an interrupted helicase domain. The regular shRNA ∼21-nt products are marked. (*) AgoshRNA ∼30-nt products. Ethidium bromide staining of small rRNAs and tRNAs are shown as loading controls below the blot. (B) The Dicer and Ago2 cleavage products were quantified using ImageQuant. Total cleavage was set at 100%.