FIGURE 5.

Synthetic miR-146a represses endogenous COX-2 protein in A549 cells. A549 cells were transiently transfected with synthetic miRNAs (x-axis; No miR, miR-26b, miR-146a, miR-433). Cells were lysed and proteins were isolated 48 h post-transfection. (A,B) Western blot analysis was performed on cell lysates. GAPDH served as a loading control. (A) 100 nM miR; (B) 50 nM miR. (C) Densitometric quantification of relative COX-2 protein expression in samples was performed and normalized to GAPDH protein. Each transfection was performed in triplicate in independent experiments.