Skip to main content
. 2014 Sep;20(9):1451–1464. doi: 10.1261/rna.046144.114

FIGURE 5.

FIGURE 5.

Primary and secondary structures of other kissing-loop interactions considered in this study. (A) The HIV-1 TAR/TAR* kissing-loop complex used for the present study. Similar complexes were used in previous thermodynamic studies of the TAR/TAR* complex (Ducongé et al. 2000; Nair et al. 2000). (B) The ColE1 RNAIi/RNAIIi complex previously thermodynamically characterized by UV melting curves (Gregorian and Crothers 1995). (C) The HIV-1 DIS complex (XLAI/YLAI) previously investigated by UV melting studies (Weixlbaumer et al. 2004). (D) The MMLV H3-18 kissing-loop complex used here and in previous UV melting studies (Kim and Tinoco 2000). In A–D, interactions at the kissing-loop junction are highlighted by pale gray shading for W-C base pair and dark gray boxes for base stacking, according to previously determined three-dimensional structures of the HIV-1 TAR/TAR* (pdb code 1KIS.pdb) (Chang and Tinoco 1997), ColE1 RNAIi/RNAIIi (pdb code 1BJ2) (Lee and Crothers 1998), HIV-1 DIS (pdb code 2B8R) (Ennifar et al. 2001), and MMLV H3-18 kissing-loop complexes (pdb code 1F5U) (Kim and Tinoco 2000).