FIGURE 2.

Lsm1-7 complex is severely impaired in its ability to bind RNA and does not exhibit a binding preference for oligoadenylated RNA. BSA or increasing concentrations of Lsm1-7–Pat1 complex purified from wild-type cells or the Lsm1-7 complex purified from pat1Δ cells (indicated on top in A and B) were subjected to gel shift assays using uniformly radiolabeled PGK1 and PGK1-A₅ RNAs (A) or MFA2 and MFA2-A₅ RNAs (B). Plots of the percentage of RNA bound vs. the concentration of the complex used are shown on the right of the phosphorimages of the gels. Bands of bound and unbound RNA are labeled.