Figure 3.

Maximum-likelihood phylogenetic tree of sequences affiliated with Janthinobacterium including the environmental clones retrieved from Arctic seawater (highlighted in bold) from the clone libraries constructed with DNA from different size-fractions. Janthinobacterium representatives with their genomes sequenced have also been highlighted in bold, and those isolated from cold environments (including ice, snow, glacier melt waters and high-mountain lakes) appear in blue. Isolation sources are shown to the right. The tree was rooted with a clade containing sequences of Burkholderia spp. (full tree is shown in Supplementary Figure 4), and bootstrap values based on 1000 replicates (>50%) are indicated on the branches. The pie charts show the proportion of clones affiliated with different bacterial phylogenetic groups as identified by the RDP classifier in the small and large size-fraction clone libraries (Verruco: Verrucomicrobia, Alpha: Alphaproteobacteria, Beta: Betaproteobacteria, Gamma: Gammaproteobacteria, Delta: Deltaproteobacteria, Janthino: Janthinobacterium).