Fig. 6.
RNAi-mediated knockdown of Croquemort transcript SCRBQ2 in the A. gambiae mosquito. Real-time quantitative PCR analysis of the SCRBQ2 Croquemort transcript expression in the (A) midguts and (B) carcasses of A. gambiae females injected with dsSCRBQ2 or dsGFP RNA. Samples were obtained 48, 72 and 96 h after dsRNA injection and analyzed by real-time RT-PCR. (C) Croquemort expression in the midguts of A. gambiae female mosquitoes injected with dsSCRBQ2 or dsGFP and fed 72 h later with P. berghei-infected blood. Samples were obtained 96 h after dsRNA injection (24 h after infectious-blood feeding). The A. gambiae S7 ribosomal protein mRNA served as a quantification standard. Bars indicate 95% confidence intervals.