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. 2014 Aug 20;4:6138. doi: 10.1038/srep06138

Figure 4. The effect of sULBP3 on NK cell activity.

Figure 4

A549 (A), K562 (B), 7721 (C), and CD133−SW620 (D) cells were co-cultured with NK cells, with or without the indicated concentrations of ULBP3-Fc. NK cell cytotoxicity was measured by LDH release after co-culture for 6 h at an E:T ratio of 10:1. A549 (E), K562 (F), 7721 (G), and CD133−SW620 (H) were co-cultured with NK cells respectively at the indicated E:T ratios, with or without adding high (30 ng/ml) or low (1 ng/ml) concentration of ULBP3-Fc. The NK cell cytotoxicity was measured by LDH release after co-culture for 6 h. Data are representative of the results obtained from at least three independent experiments. Results are expressed as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.