Table 3.

Subcellular localization of Arabidopsis thaliana Cys-GST members and of poplar orthologs.

Gene name	Accession number	Predicted subcellular localization	Confirmed localization and other proteomic evidence	Amino acid length	Orthologs in poplar	References
DHAR1	At1g19570	Elsewhere	- FP fusions: cytosola, peroxisomeb - High-throughput proteomic: mitochondriac, cytosold, plasma membranee, chloroplastf	213	- FP fusions: DHAR2: cytosolb, DHAR3: cytosolb	aGrefen et al., 2010 bReumann et al., 2009 cChew et al., 2003 dIto et al., 2011 eMarmagne et al., 2007 fPeltier et al., 2006
DHAR2	At1g75270	Elsewhere	- High-throughput proteomic: cytosola, plasma membraneb	213	- FP fusions: DHAR2: cytosolb, DHAR3: cytosolb	aIto et al., 2011 bMarmagne et al., 2007 cTang and Yang, 2013
DHAR3	At5g16710	Chloroplast	- High-throughput proteomic: chloroplasta	258	- FP fusions: DHAR1: chloroplastb	aZybailov et al., 2008 bTang and Yang, 2013
GSTL1	At5g02780	Elsewhere	None	237	- FP fusions: GSTL2, GSTL3A & B: nucleocytoplasmica	aLallement et al., 2014
GSTL2	At3g55040	Chloroplast	- FP fusions: peroxisomea, - High-throughput proteomic: chloroplastb-d	292	- FP fusions: GSTL1: chloroplaste	aDixon et al., 2009 bZybailov et al., 2008 cPeltier et al., 2006 dFerro et al., 2010 eLallement et al., 2014
GSTL3	At5g02790	Elsewhere	- High-throughput proteomic: cytosola	235	- FP fusions: GSTL2, GSTL3A & B: nucleocytoplasmicb	aIto et al., 2011 bLallement et al., 2014
GHR1	At4g19880	Chloroplast	- High-throughput proteomic: cytosola, chloroplastb	356		aIto et al., 2011 bKlodmann et al., 2011
GHR2	At5g45020	Elsewhere	None	325
GHR3	At5g44990	Elsewhere	None	350
GHR4	At5g44000	Chloroplast	- High-throughput proteomic: chloroplasta	399		aFerro et al., 2010
mPGES-2	At5g42150	Mitochondria or chloroplast	- High-throughput proteomic: mitochondriaa,b, plasma membranec	315		aHeazlewood et al., 2004 bKlodmann et al., 2011 cMitra et al., 2009

The prediction of subcellular localization was performed by compiling results obtained from various softwares as Predotar, TargetP, and Wolfpsort. Experimental confirmation consisting of high-throughput proteome analyses and fusions with fluorescent proteins (FP fusions) and associated references are indicated when available.