Table 4.

Tridimensional structures of Cys-GSTs from all kingdoms.

Class	Name	Organism	Ligand 1	Ligand 2	PDB	References
?	TDR1	L. infantum	GSH	1,2-Ethanediol	4AGS	Fyfe et al., 2012
?	LigG	S. paucimobilis	GSH	SO2-4; Acetate	4G10	Meux et al., 2012
GSTB	BphK	B. xenovorans	GSH	2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid	2DSA	Tocheva et al., 2006
GSTB		E. coli	GTS	–	1A0F	Nishida et al., 1998
GSTB		M. haemolytica	GSH	Triethylene glycol; Cl−; Acetate	4IW9	Unpublished
GSTB		M. capsulatus	GSH	Glycerol	3UAR	Unpublished
GSTB		O. anthropi	GSH	SO2-4	2NTO	Federici et al., 2007
GSTB		P. mirabilis	GSH	–	1PMT	Rossjohn et al., 1998
GSTB		S. flexneri	GSH	–	4KGI	Unpublished
GSTB		S. paucimobilis	GSH	–	1F2E	Unpublished
GSTB		X. fastidiosa	GSH	Cl−	2X64	Unpublished
GSTB		Y. pestis	GSH	Glycerol	4G9H	Unpublished
CLIC	EXC-4	C. elegans	–	Ca2+	2YV9	Littler et al., 2008
CLIC		D. melanogaster	–	Ca2+; I−	2YV7	Littler et al., 2008
CLIC	CLIC1	H. sapiens	GSH	–	1K0N	Harrop et al., 2001
CLIC	CLIC4	H. sapiens	–	–	2AHE	Littler et al., 2004
CLIC	CLIC2	H. sapiens	GSH	–	2R4V	Cromer et al., 2007
CLIC	CLIC3	H. sapiens	–	SO2-4	3FY7	Littler et al., 2010
GRX	Grx2	E. coli	–	–	1G7O	Xia et al., 2001
GRX	Grx2	S. enterica	GSH	SO2-4; Cl−	3IR4	Unpublished
GSTO*	GSTO3-3	B. mori	–	Glycerol	3RBT	Chen et al., 2011
GSTO	GSTO1-1	H. sapiens	GSH	SO2-4	1EEM	Board et al., 2000
GSTO	GSTO2-2	H. sapiens	GSH	Cl−	3Q19	Zhou et al., 2012
mPGES-2		M. fascicularis	–	Indomethacin; Cl−; Acetate	1Z9H	Yamada et al., 2005
GHR	YqjG	E. coli	–	GS-menadione	4G0K	Green et al., 2012
GHR		C. glutamicum	–	1,2-Ethanediol; Glycerol	3M1G	Unpublished
GHR		P. chrysosporium	GSH	–	3PPU	Meux et al., 2011
GHR	PcpF	S. chlorophenolicum	–	–	4FQU	Green et al., 2012
GHR		G. bronchialis	–	–	4PTS	Unpublished
GSTL	GSTL3	P. trichocarpa	GSH	Ca2+	4PQI	Lallement et al., 2014
GSTL	GSTL1	P. trichocarpa	GSH	Na+	4PQH	Lallement et al., 2014

Available Cys-GST structures have been retrieved from the RCSB Protein data bank (http://www.rcsb.org/pdb/home/home.do). Only the first solved structures of wild-type isoforms have been listed, but for several proteins as human CLIC or GSTOs or E. coli YqjG, other structures have been obtained either for mutated proteins or for wild-type proteins in complex with GSH or another second ligand. For instance, in the case of E. coli YqjG, there are 3 structures described in the same study, one in apoform, one with GSH and one with GS-menadione. Beyond GSH, structures of Cys-GSTs with physiological substrates have been obtained in rare cases. The compounds indicated in the ligand 2 column essentially come from crystallization solutions.

^*GSTO3-3 from B. mori is phylogenetically related to and classified as GSTOs although it displays an asparagine instead of the catalytic cysteine. GTS is for glutathione sulfonate.