Aspirin (975mg) was administered 1hour prior to commencement of the study. In separate protocols, FBF measurements were performed after either intra-arterial infusions of bradykinin (100, 200 and 400 ng/min), or intra-arterial acetylcholine (7.5, 15 and 30 μg/min), or handgrip exercise (15%, 30% and 45% of maximum grip strength) followed by endothelium-independent vasodilation with sodium nitroprusside (1.6, 3.2 μg/min). Measurements were repeated after NO blockade with L-NMMA, K+Ca channel blockade with TEA, and after combined blockade with L-NMMA and TEA.