Table 2. Expression levels of KMTs in the hippocampus.
Quantitative PCR was used to determine expression of H3K9 and H3K27 KMTs relative to expression of the housekeeping gene Gapdh in the control hippocampus of adult male mice. One-way ANOVA was used to assess for change in expression over time following pilocarpine-induced seizure.
| Gene symbol | Synonyms | Histone substrate | % of Gapdh | F(3,19) | p-value |
|---|---|---|---|---|---|
| Suv39h1 | KMT1A | H3K9 | 0.6265 | 0.562 | 0.65 |
| Suv39h2 | KMT1B | H3K9 | 0.1516 | 6.317 | 0.019* |
| Ehmt1 | KMT1D/GLP | H3K9, H1.4K26 | 0.5717 | 1.549 | 0.24 |
| Ehmt2 | KMT1C/G9a | H3K9, H1.4K26 | 6.1235 | 1.012 | 0.41 |
| Setdb1 | KMTIE | H3K9 | 0.6010 | 2.134 | 0.14 |
| Kmt2a | Mll1 | H3K4 | 0.8498 | 0.772 | 0.53 |
| Kmt2b | Mll2 | H3K4 | 0.2941 | 0.839 | 0.49 |
| Kmt2c | Mll3 | H3K4 | 0.2755 | 1.341 | 0.3 |
| Ezh1 | KMT6B | H3K27 | 0.7335 | 0.976 | 0.43 |
| Ezh2 | KMT6A | H3K27, H1.4K26 | 0.2959 | 7.468 | 0.04* |
*
p<0.05.
Dark grey highlighting denotes two KMTs that increased in expression after pilocarpine treatment.