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. 2014 Aug 20;5:308. doi: 10.3389/fphys.2014.00308

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Copyright © 2014 Garcia and Larina.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hemodynamic analysis in the embryonic yolk sac. (A) Cultured embryos with erythrocytes labeled using the ε-globin-GFP transgene near the blood islands at E8.5. (B) Tracks of all cells detected using image processing software during the movie represented in (A). The colored lines represent the position of the blood cells over the course of the time lapse. (C) Plot of individual cell velocities over the course of the time lapse. This allows for the quantification of average blood velocity, peak blood velocity, velocity profiles, and heart rate.