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. 2014 Jun 27;155(9):3251–3261. doi: 10.1210/en.2014-1002

Figure 2.

Figure 2.

Impact of SMRT depletion on growth of antiestrogen-treated MCF-7 cells. Cells were transfected with nontargeting control (siCtrl) or SMRT-specific (siSMRT) siRNA and treated with vehicle or 500 nM ICI 182,780 (ICI) to block ERα activity. The next day cells were treated with vehicle or 1 nM E2 and after 5 days of culture, harvested for cell number determinations. Data presented is average ± SEM of three experiments normalized to vehicle-treated siCtrl cells. Statistical analyses were performed with Student's t test; #, P < .05 versus vehicle treatment within the same siRNA group and *, P < .05 versus the corresponding ligand treatment in the siCtrl group.