Figure 3.

Decreased Paneth and goblet cells but no change in EEC in DIO mice vs controls. A, C, and E, Representative immunofluorescent images of jejunal sections stained with (A) lysozyme (Lyz), (C) mucin2 (Muc2), or (E) Chromogranin-A (ChgA). B, D, and F, Quantification of (B) Lyz positive Paneth cells, (D) Muc2 positive goblet cells, and (F) ChgA positive EEC in DIO vs control mice. All images were taken at 20x magnification. Scale bar, 50 μm. Data expressed as mean ± SEM. *, P < .05 DIO vs control, unpaired t test, n ≥ 4.