Figure 4.
Sox9-EGFPLow ISC from DIO mice are enriched for appropriate biomarkers and show elevated cyclin D1 mRNA. High throughput qRT-PCR on FACS isolated Sox9-EGFP cells from control and DIO mice assessed levels of mRNAs encoding (A) EGFP, (B) Sox9, (C) Chga, (D) Hopx, (E) Lct, (F) Lgr5, and (G) Ccnd1. Data expressed as mean ± SEM. * or **, P < .05 vs all other Sox9-EGFP populations; #, P < .05 in DIO vs control Sox9-EGFPLow ISC. Differences between Sox9-EGFP populations compared by 1-way ANOVA, Holm-Sidak and between diets by paired t test, n ≥ 3.
