Figure 5.

Sox9-EGFP^Low ISC from DIO mice exhibit reduced enteroid-forming ability. A, Time course of enterosphere/enteroid formation by ISC isolated from control and DIO mice. Images were taken at 10x magnification. Scale bar, 100 μm. B, Quantification of enterosphere/enteroids formed starting at day 4 until day 12. Data expressed as mean percentage vs enteroids formed in control at day 4 ± SEM. *, P < .05 DIO vs control, paired t test, n ≥ 6 independent pairs performed in duplicate or triplicate.