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. 2014 Jun 10;155(9):3302–3314. doi: 10.1210/en.2014-1112

Figure 6.

Figure 6.

Plasma insulin positively correlates with percentage of Sox9-EGFPLow ISC and treatment of ISC from DIO mice with insulin, IGF1, or both rescues decreased intrinsic in vitro function. A, Linear regression analysis of plasma insulin levels and percentage of Sox9-EGFPLow ISC in individual mice. B, Enteroid formation at day 12 from Sox9-EGFPLow ISC isolated from control or DIO mice cultured with standard growth factors (Std. GF) as described in Materials and Methods or Std. GF plus insulin (Ins.), IGF1, or Ins. and IGF1. Data expressed as mean ± SEM. *, P < .05 vs control cultured with Std. GF alone; **, P < .05 vs DIO Std. GF alone, paired t test, n = 3 for ISC isolated from 3 independent littermate pairs each cultured in duplicate. C and D, High throughput qRT-PCR measured (C) Igf1r and (D) total Ir mRNA. E, RT-PCR on Sox9-EGFPLow ISC measured IR isoform expression. F, Ratio of IR-A to IR-B expression quantified in control vs DIO Sox9-EGFPLow ISC. Data expressed as mean ± SEM. *, P < .05 vs all Sox9-EGFP cell populations, 1-way ANOVA, Holm-Sidak, n ≥ 3.