Table 6.

PKA Subunit mRNA and Protein Expression in Liver of WT and RIIαKO Mice After 14 Weeks of ad Libitum Feeding With CD or HFD

	RIα	RIIα	RIIβ	Cα	Cβ
mRNA expression (qPCR)
CD
WT (n = 7)	0.99 ± 0.12	1.10 ± 0.13	1.01 ± 0.18	0.98 ± 0.09	1.02 ± 0.13
Mutant (n = 7)	0.98 ± 0.05	0.19 ± 0.18	0.81 ± 0.15	0.72 ± 0.08	1.02 ± 0.12
HFD
WT (n = 7)	0.65 ± 0.04	0.55 ± 0.12	1.19 ± 0.27	0.60 ± 0.05	0.49 ± 0.07
Mutant (n = 6)	0.91 ± 0.06	0.02 ± 0.02	0.80 ± 0.20	0.74 ± 0.06	1.05 ± 0.26
	P = .14	P < .001	P = .17	HFD, P = .086; CD, P = .059a	P = .10
Protein expression (Western blot)
CD
WT (n = 3)	3.30 ± 0.55	2.91 ± 0.58	13.77 ± 5.35	6.39 ± 1.59	6.94 ± 0.69
Mutant (n = 3)	18.52 ± 3.89	0.28 ± 0.15	5.95 ± 2.68	1.33 ± 0.34	3.19 ± 0.30
HFD
WT (n = 3)	3.20 ± 2.55	1.71 ± 0.12	8.97 ± 4.30	5.07 ± 1.12	2.28 ± 0.44
Mutant (n = 3)	16.70 ± 6.60	0.17 ± 0.10	3.90 ± 1.89	0.87 ± 0.27	0.79 ± 0.12
	P = .005	P < .001	P = .11	P < .001	HFD, P = .032; CD, P = .007a

Abbreviation: qPCR, quantitative PCR. mRNA expression was presented as fold-change (2^−ΔΔCT); WT mice on CD served as the control. Protein expression was calculated as the ratio of protein of interest to housekeeper. Data shown are for female mice. P values derive from multivariable ANCOVA adjusting for diet; values are mean ± SEM.

^aReported separately for each diet due to statistically significant interaction with genotype.