Figure 1.
IL-1β+IFNγ (CTK) induces ER stress factors, iPLA2β, and mSREBP-1 in human pancreatic islets. Islets (500/condition) were treated with either vehicle (DMSO) or IL-1β+IFNγ (CTK), harvested at 24 and 48 hours, and processed for immunoblotting analyses for GRP78, pPERK, iNOS, iPLA2β, and mSREBP-1. Tubulin was used as loading control. A and C, Analyses were repeated 3 times and representative blots are presented. B and D, Densitometry quantitation. (*Significantly different vs corresponding DMSO groups, P < .05; §vs other groups, P < .05; # and † vs corresponding DMSO groups, P < .01 and P < .05, respectively, n = 3.)