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. 2014 Aug 20;5:438. doi: 10.3389/fmicb.2014.00438

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Copyright © 2014 Crane, Bauler, Wehrly and Bosio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mROS potentiates Fn mediated inflammasome activation. BMM were treated with mitoTEMPO or DMSO (vehicle control; mock) 1 h prior to infection with Fn MOI 250. (A) Intracellular bacteria were enumerated at the indicated times after infection. ns, not significant. (B) Western blots of cell lysates were probed for caspase-1 p20 and actin. (C) Culture supernatants were assessed for IL-1 by ELISA. Error bars depict SD. ns, no significant difference between mock treated cells. *p < 0.05 compared all mock treated cells. Data are representative of two experiments of similar design.