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Secondary PCR product was digested by VSPI restriction enzymes. Lane 1, DNA size marker. Lane 2, undigested PCR product. Lanes 3-6 and 11-13, C. parvum 628,104 bp. Lane 7, C. meleagridis 457 171 and 115 bp. Lanes 8-10, C. hominis 556 and 116 bp.
