Table 1. Primers Used in Multiplex PCR (11) .
| Family | Target (s) | Primer | Sequence (5’ to 3’, as Synthesized) | Expected Amplicon Size (bp) | Nucleotide Positions |
|---|---|---|---|---|---|
| MOX | MOX-1, MOX-2,CMY-1, | MOXMF | GCT GCT CAA GGA GCA CAG GAT | 520 | 358–378 |
| CMY-8 to CMY-11 | MOXMR | CAC ATT GAC ATA GGT GTG GTG C | 877–856 | ||
| CIT | LAT-1 to LAT-4 | CITMF | TGG CCA GAA CTG ACA GGC AAA | 462 | 478–498 |
| CMY-2 to CMY-7, BIL-1 | CITMR | TTT CTC CTG AAC GTG GCT GGC | 939–919 | ||
| DHA | DHA-1, DHA-2 | DHAMF | AAC TTT CAC AGG TGT GCT GGG T | 405 | 1244–1265 |
| DHAMR | CCG TAC GCA TAC TGG CTT TGC | 1648–1628 | |||
| EBC | MIR-1T ACT-1 | EBCMF | TCG GTA AAG CCG ATG TTG CGG | 302 | 1115–1135 |
| EBCMR | CTT CCA CTG CGG CTG CCA GTT | 1416–1396 | |||
| FOX | FOX-1 to FOX-5b | FOXMF | AAC ATG GGG TAT CAG GGA GAT G | 190 | 1475–1496 |
| FOXMR | CAA AGC GCG TAA CCG GAT TGG | 1664–1644 |