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. Author manuscript; available in PMC: 2014 Aug 20.
Published in final edited form as: J Neuroimmunol. 2010 Jun;223(0):55–64. doi: 10.1016/j.jneuroim.2010.04.002

Fig. 4.

Fig. 4

Effect of CS-A and CSPG-DS on IL-6, IL-17 and IFN-γ production in splenocytes and brain-infiltrating cells from EAE mice. EAE induction and treatment with CS-A or CSPG-DS were carried out as described in Fig 1. Without re-stimulation in vitro, splenocytes from control mice, which did not receive MOG35-55 immunization and Pertussis toxin, and EAE mice untreated or treated with CS-A or CSPG-DS, on day 14, were stained for intracellular expression of: (A) IL-6; (B) IL-17; and (C) IFN-γ. IFN-γ production in the brain-infiltrating cells after in vitro stimulation was also determined by ELISA from EAE mice untreated or treated with CS-A or CSPG-DS on day 14 (D). Data were presented as mean ± SEM (error bars) of multiple experiments from three mice/group.