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. Author manuscript; available in PMC: 2014 Nov 22.
Published in final edited form as: Nature. 2014 Apr 9;509(7501):492–496. doi: 10.1038/nature13180

Extended Data Figure 3. Identification of Cu-binding mutants of MEK1 that reduce ERK1/2 phosphorylation.

Extended Data Figure 3

a, Immunoblot detection of the amount of HA-tagged wild-type (WT) MEK1 and an example of one MEK1 mutant tested (H188A) that bound to a Cu-charged resin. Input serves as a loading control. b, Immunoblot detection of the amount of phosphorylated (P) and/or total (T) ERK1/2 or HA-MEK1 protein in immortalized Ctr1+/+ MEFs stably expressing HA-tagged wild-type (WT) MEK1 or an example of one MEK1 mutant tested (H188A). c, Summary of whether the indicated MEK1 point mutants did (YES) or did not (NO) exhibit a reduction in binding to the Cu-charged resin or show a reduction in the levels of phosphorylated (P) ERK1/2 when stably expressed in immortalized Ctr1+/+ MEFs. Gel images are representative of two replicates.