Figure 2.

Assignment of LSm5 in the LSm2–8 complex. A) LSm5 in the LSm2–8 complex before (black) and after (pink) the addition of the 3′ end of the U6 snRNA B) LSm5 resonance strips from an HNCA experiment that was recorded on a fully labeled LSm2–8 complex. The resonance strips could be selected based on the LSm5 LEGO-NMR spectrum (A, and Figure 1 C). C) LSm5 residues that experience the largest chemical shift changes upon addition of the U6 snRNA are marked as pink spheres on a model of the LSm2–8 complex.