Figure 4.

Mixed ¹⁵N, ε-¹H,¹³C-methyl methionine and δ1-¹H,¹³C-methyl isoleucine labeling of LSm2–8. The ¹H-¹⁵N TROSY spectrum (left) displays all subunits in the complex (see Figure 1 B). LSm2, LSm3, LSm4, and LSm8 are labeled with methionine residues (see Figure 3 A, that displays the methionine methyl groups for LSm1–7). LSm5, LSm6, and LSm7 are labeled with isoleucine residues (see Figure 3 B).