Figure 2.

Dyngo compounds are potent, reversible inhibitors of endocytosis in non‐neuronal cells. A) The effect of three Dyngo analogs 4a, 6a and 1a on endocytosis was compared with that of Dynasore (synthesized in‐house) by examining Tfn‐A594 uptake in U2OS cells. B and C) The time required for 10 μM 4a (B) and 6a (C) to inhibit non‐neuronal CME. Dyngo compounds were preincubated with cells before performing a Tfn uptake assay. D and E) Reversibility of endocytosis inhibition. The 10 μM 4a (D) and 6a (E) were incubated with cells for 30 min and then removed. Tfn uptake was then quantified at the indicated times after removal of the compound (washout time). Data are mean ± SEM of at least three independent experiments.